Coding

Part:BBa_K1680008:Design

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)

Δ1-19 Cre recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 258


Design Notes

The Part is intended for use with the RFC25 standard. Because this standard already contains both a start and a stop codon in the prefix/suffix, the part only sequence does NOT contain these. This part is intended for generation of Cre fusion proteins. The first 19 amino acids of the Cre recombinase were removed because these amino acids are most likely very flexible, which is indicated by the fact they could not be crystallized when the structure was determined (RCSB: 3MGV) [http://dx.doi.org/10.2210/pdb3mgv/pdb].

Source

The original sequence of Cre recombinase can be found in uniprot [http://www.uniprot.org/uniprot/P06956]. This sequence was then codon optimized for expression in Saccharomyces cerevisiae and compability with RFC25 standard by Team Tuebingen.


References